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bsk6 slice keeper  (Scientific Systems Design Inc)


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    Structured Review

    Scientific Systems Design Inc bsk6 slice keeper
    Bsk6 Slice Keeper, supplied by Scientific Systems Design Inc, used in various techniques. Bioz Stars score: 93/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/brain+slice+keeper/Brain+Slice+Keeper+1+and+Vessel/pm40355868-41-7-10
    Average 93 stars, based on 23 article reviews
    bsk6 slice keeper - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Slice Preparation:

    Article Title: Nicotine and electronic cigarette (E-Cig) exposure decreases brain glucose utilization in ischemic stroke
    Article Snippet: Using a Leica VT1000 S Vibrating blade microtome (Leica Biosystems, Wetzlar, Germany; Cat# 14047235613), 200-μm thin coronal slices were cut from each hemisphere after discarding 1.2 mm brain area rostrally. .. Slices were kept in a brain slice keeper (Scientific Systems Design Inc., Mississauga, Ontario, Canada; Cat# S-BSK4) immersed in HEPES buffer with continuous oxygen perfusion at 37°C. ..

    Article Title: Arctic ground squirrel hippocampus tolerates oxygen glucose deprivation independent of hibernation season even when not hibernating and after ATP depletion, acidosis and glutamate efflux
    Article Snippet: .. The slices were then transferred to a brain slice keeper (Scientific Systems Design Inc., Mississauga, Ontario, CA) and allowed to recover for 1–1.5 h at room temperature (20–21°C) in HEPES-aCSF bubbled continuously with 95% O 2 /5% CO 2 before transferring to microperfusion chambers. ..

    Article Title: Arctic Ground Squirrel resist peroxynitrite-mediated cell death in response to oxygen glucose deprivation
    Article Snippet: Rapidly dissected hippocampi were embedded in 2.5 % agar and transverse hippocampal slices, 400 μm thick were cut at approximately 2°C in oxygenated HEPES-aCSF using a Vibratome® 1000plus sectioning system (The Vibratome Company, St. Louis, MO). .. The slices were then transferred to a brain slice keeper (Scientific Systems Design Inc., Mississauga, Ontario, CA) and allowed to recover for 1–1.5 h at room temperature (20–21°C) in HEPES-aCSF bubbled continuously with 95% O 2 /5% CO 2 before transferring to microperfusion chambers. ..

    Article Title: Nicotine and electronic cigarette (E-Cig) exposure decreases brain glucose utilization in ischemic stroke.
    Article Snippet: Using a Leica VT1000 S Vibrating blade microtome (Leica Biosystems, Wetzlar, Germany; Cat# 14047235613), 200-lm thin coronal slices were cut from each hemisphere after discarding 1.2 mm brain area rostrally. .. Slices were kept in a brain slice keeper (Scientific Systems Design Inc., Mississauga, Ontario, Canada; Cat# S-BSK4) immersed in HEPES buffer with continuous oxygen perfusion at 37°C. ..

    Transferring:

    Article Title: Arctic ground squirrel hippocampus tolerates oxygen glucose deprivation independent of hibernation season even when not hibernating and after ATP depletion, acidosis and glutamate efflux
    Article Snippet: .. The slices were then transferred to a brain slice keeper (Scientific Systems Design Inc., Mississauga, Ontario, CA) and allowed to recover for 1–1.5 h at room temperature (20–21°C) in HEPES-aCSF bubbled continuously with 95% O 2 /5% CO 2 before transferring to microperfusion chambers. ..

    Article Title: Arctic Ground Squirrel resist peroxynitrite-mediated cell death in response to oxygen glucose deprivation
    Article Snippet: Rapidly dissected hippocampi were embedded in 2.5 % agar and transverse hippocampal slices, 400 μm thick were cut at approximately 2°C in oxygenated HEPES-aCSF using a Vibratome® 1000plus sectioning system (The Vibratome Company, St. Louis, MO). .. The slices were then transferred to a brain slice keeper (Scientific Systems Design Inc., Mississauga, Ontario, CA) and allowed to recover for 1–1.5 h at room temperature (20–21°C) in HEPES-aCSF bubbled continuously with 95% O 2 /5% CO 2 before transferring to microperfusion chambers. ..

    other:

    Article Title: Combinatorial targeting of distributed forebrain networks reverses noise hypersensitivity in a model of autism spectrum disorder
    Article Snippet: Slices were then incubated in normal aCSF (containing in mM: 125 NaCl, 25 NaHCO 3 , 2.5 KCl, 1.25 NaH 2 PO 4 , 1.2 MgCl 2 , 2 CaCl 2 , 25 glucose, 1.7 L(+)-ascorbic acid, 310 mOsm) for 45 minutes at 34 °C in a Brain Slice Keeper ( Scientific Systems Design Inc. ) and kept at room temperature thereafter.



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    Image Search Results


    Reagents and tools table

    Journal: The EMBO Journal

    Article Title: The TrkC-PTPσ complex governs synapse maturation and anxiogenic avoidance via synaptic protein phosphorylation

    doi: 10.1038/s44318-024-00252-9

    Figure Lengend Snippet: Reagents and tools table

    Article Snippet: Acute 400 μm slices containing dorsal hippocampus were obtained using a VT1200S vibratome (Leica Biosystems) and then transferred to an incubation chamber (BSK12, Scientific Systems Design) containing a standard artificial cerebral spinal fluid (ACSF) comprised of (in mM): 124 NaCl, 10 d -glucose, 26 NaHCO 3 , 2.5 KCl, 1.25 NaH 2 PO 4 , 2 CaCl 2 and 1 MgSO 4 at pH of 7.3–7.4 and osmolarity of 290–300 mOsm/L.

    Techniques: Knock-In, Recombinant, Plasmid Preparation, Clone Assay, Staining, Sequencing, DC Protein Assay, Membrane, Western Blot, Electron Microscopy, Mass Spectrometry, Phospho-proteomics, Software, Fluorescence, Microscopy, Incubation